The effect of activated charcoal dose and benzyl amino purine concentration on the growth of orchid plantlets in murashige and skoog media in vitro

International Conference on Agribussines, Food and Agro-Technology

Sipayung, Patricius^1 ; Matanari, Jawaller^1 ; Lafau, Meryana Br^1 ; Sulastri, Yustina Sri^1 ; Ginting, Benny B.^1 ; Sihombing, Dewi Restuana^1 ; Pandiangan, Maruba^1 ; Giawa, Tulusman^1

Faculty of Agriculture, Universitas Katolik Santo Thomas, Indonesia^1

关键词: Completely randomized designs;    In-vitro;    Indonesia;    Murashige and skoog;    Plantlets;    Root length;    Root number;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/205/1/012025/pdf
DOI  :  10.1088/1755-1315/205/1/012025

来源: IOP

【 摘 要 】

Indonesia has a high variety of orchids. In vitro propagation of orchids was strongly influenced by the composition of the medium used. This study aims to conduct the effect of activated charcoal and BAP concentration on the growth of orchid subcultures in Murashige and Skoog Media. The experimental design used was a Completely Randomized Design (CRD) with two factors. Benzyl Amino Purine concentration (0 ml / l, 0.25 ml / l, 0.5 ml / l, and 0.75 ml / l active dose of charcoal (0 (0 g / l, 1 g / l, and 1, 5 g / l). The results showed that BAP concentrations up to 0.75 ml / l at the age of 8 mst had a significant effect on root length, but did not significantly affect the number of leaves, root number, and plantlet height. The activated charcoal up to 1.5 g / l does not have a significant effect on the number of leaves and number of sprouts at the age of 2-7 mst, but at the age of 8 mst was significantly affect the number of roots, root length, and plantlet height.