Effect of utilization of tomato extract and foliar fertilizer as media on shoots multiplication of banana cv Ambon in vitro

4th International Conference on Sustainable Agriculture and Environment

农业科学;生态环境科学

Bidhari, L.A.^1 ; Purwanto, E.^2 ; Yunus, A.^2

Departement of Agronomy, Graduate School of Universitas Sebelas Maret, Surakarta, Central Java

57126, Indonesia^1

Departement of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Central Java, Surakarta, Central Java

57126, Indonesia^2

关键词: Average length;    Completely randomized designs;    In-vitro;    Leaf length;    Media types;    Root length;    Root number;    Two ways;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/142/1/012046/pdf
DOI  :  10.1088/1755-1315/142/1/012046

来源: IOP

【 摘 要 】

The good quality banana seeds are still difficult to obtain. There are two ways to provide seeds, namely conventional and tissue culture (in vitro). Tomato extract contains natural ZPT or phytohormone which can be utilized in modification of banana tissue culture media. The aim of this study was to determine the influence of media types and tomato extracts in various concentrations for multiplication of banana cv. Ambon in vitro. The study was conducted from October - December 2016 at the Tissue Culture Laboratory of Horticulture Seed Center, Salaman, Magelang. The experimental design used was completely randomized design with two treatment factors. The firs factor was media type with the addition of foliar fertilizer, the second factor was modification of tomato extract with 4 levels. The results showed that the different of the treated media treatment did not affect the emerge of leaf and leaf length, the number of roots and root length. The emerge of the leaves of all treatments occurred at 6 days after planting with the highest average length was obtained in MS treatment with a combination of tomato extract 50 ml/l (10.3 cm). The use of MS medium with a combination of tomato extract 50 ml/l generated the average root number 15.5 with a root lengths 7.5 cm. Substitution of MS medium with tomato extract and foliar fertilizer did not show better results compared to the use of MS media in the multiplication of banana shoots in tissue culture.