FTIR and Proteome Analysis of Sunflower Seedling (Helianthus annuus L.) under Lead Stress

2018 1st International Conference on Environment Prevention and Pollution Control Technology

生态环境科学

Wang, Yushuang^1 ; Yang, Renjie^2 ; Ding, Lu^1 ; Xie, Xiaodong^1 ; Liu, Haixue^1,3,4

College of Agronomy and Resources and Environment, Tianjin Agricultural University, Tianjin, China^1

College of Engineering and Technology, Tianjin Agricultural University, Tianjin, China^2

Laboratory of Agricultural Analysis, Tianjin Agricultural University, Tianjin, China^3

Resource and Environmental Sciences, Tianjin Agricultural University, Jinjing Road No. 22, Tianjin

300384, China^4

关键词: Dynamic changes;    Helianthus annuus;    MALDI-TOF/TOF MS;    Protein expressions;    Protein secondary structure;    Protein spots;    Proteome analysis;    Two-dimensional (2D) electrophoresis;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/199/3/032064/pdf
DOI  :  10.1088/1755-1315/199/3/032064

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The effects of lead stress on the dynamic changes of protein secondary structure and differential protein expression of sunflower seedlings in soil were studied using Fourier transform infrared spectroscopy (FTIR) and two-dimensional (2D) electrophoresis. Under lead stress treatment, the α-helices and β-sheets of protein secondary structure for sunflower seedlings were changed. The content changes, 110% increased in a-helices of roots and 40% declined in β-sheets of leaves, were observed, respectively. Furthermore, 11 differential protein spots were obtained by analyzing 2-DE gel images, while No. 9 were identified by MALDI-TOF/TOF MS, successfully.