Improved Detection and Fragmentation of Disulphide-Linked Peptides

Methods and Protocols

Clerens, Stefan¹  Maes, Evelyne²  Dyer, Jolon M.³  Deb-Choudhury, Santanu⁴ 

[1] Biomolecular Interaction Centre, University of Canterbury, Christchurch 8140, New Zealand;Food & Bio-Based Products, AgResearch Ltd., 1365 Springs Road, Lincoln 7674, New Zealand;Riddet Institute, Massey University, Palmerston North 4442, New Zealand;Wine, Food & Molecular Biosciences, Lincoln University, Lincoln 7674, New Zealand

关键词: disulphide bonds;    MALDI-TOF/TOF MS;    aniline;    proof-of-existence;   

DOI  :  10.3390/mps1030033

学科分类：生物技术

来源: mdpi

【 摘 要 】

Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required.

【 授权许可】

CC BY