Anti-oxidative and protective effect of soy tempeh on di(2-ethylhexyl)-phthalate (DEHP) injured FL83B mice liver cells

2nd International Conference on Eco Engineering Development 2018

生态环境科学

Lo, Diana^1 ; Rawendra, Reynetha D.S.^1 ; Huang, Cian-Song^2 ; Khatri-Chhetri, Rupak^3 ; Wang, Yuh-Tai^4 ; Wu, Ming-Chang^2

Food Technology Department, Faculty of Engineering, Bina Nusantara University, Jakarta

11480, Indonesia^1

Department of Food Science, National Pingtung University of Science and Technology, No. 1, Hsueh Fu Road, Nei-Pu Hsiang, Pingtung

91201, Taiwan^2

G.P. Koirala College of Agriculture and Research Center, Faculty of Science and Technology, Purbanchal University, Nepal^3

Life Science Center, Hsing Wu University, LinKou District, New Taipei City

24452, Taiwan^4

关键词: Antioxidant capacity;    Di-2-ethylhexyl phthalate;    Hepatoprotective activities;    Lactobacillus acidophilus;    Lactobacillus bulgaricus;    Lactobacillus plantarum;    Phenolic compounds;    tempeh;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/195/1/012058/pdf
DOI  :  10.1088/1755-1315/195/1/012058

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The interest on Tempeh, a well known Rhizopus oligosporus-fermented food from Indonesia, is keep increasing as it possess potential bioactivities. Previous research on co-inoculation of Rhizopus oligosporus together with lactic acid bacteria in tempeh making showed promising results as lactic acid bacteria did not inhibit the growth of Rhizopus oligosporus. The aims of this study are to analyze the bioactivities (antioxidative and hepatoprotective activities) of single- and co-inoculated tempeh compared to pre-fermented soybean. Experiments was conducted by observing the effect of different lactic acid bacteria (Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei) on antioxidant capacities. Afterwards, one of lactic acid bacteria strain will be used to produce tempeh and the protective activity against di(2-ethylhexyl)-phthalate (DEHP)-injured FL83B cell was observed. The result showed that Lactobacillus plantarum co-inoculated tempeh had the highest ferrous ion chelating activity, while Lactobacillus casei co-inoculated tempeh had the highest radical scavenging acticity and reducing power. Moreover, genistein and daidzein, aglycone isoflavone, found highest in Lactobacillus bulgaricus co-inoculated tempeh. For hepatoprotective activity, Lactobacillus plantarum co-inoculated and single-inoculated tempeh had protective activities against DEHP-injured FL83B cell, while soybean has no significant protective effect.