New strains of oil-degrading microorganisms for treating contaminated soils and wastes

3rd International Conference Environment and Sustainable Development of Territories: Ecological Challenges of the 21st Century

生态环境科学;地球科学;经济学

Muratova, A.Yu.^1 ; Panchenko, L.V.^1 ; Semina, D.V.^1 ; Golubev, S.N.^1 ; Turkovskaya, O.V.^1

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov, 13, Saratov

410049, Russia^1

关键词: Achromobacter;    Acinetobacter sp;    Ammonium nitrate;    Bioremediation agent;    Contaminated soils;    Oil contaminated soil;    Soil microflora;    Total petroleum hydrocarbons;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/107/1/012066/pdf
DOI  :  10.1088/1755-1315/107/1/012066

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Two new strains Achromobacter marplatensis101n and Acinetobacter sp. S-33, capable of degrading 49 and 46% of oil within 7 days were isolated, identified, and characterized. The application of A. marplatensis 101n in combination with ammonium nitrate (100 mg•kg-1) for 30 days of cultivation resulted in the degradation of 49% of the initial total petroleum hydrocarbon content (274 g•kg-1) in the original highly acid (pH 4.9) oil-contaminated waste. Up to 30% of oil sludge added to a liquid mineral medium at a concentration of 15% was degraded after 10 days of cultivation of A. marplatensis 101n. Application of yellow alfalfa (Medicago falcata L.) plants with Acinetobacter sp. S-33 for bioremediation of oil-sludge-contaminated soil improved the quality of cleanup in comparison with the bacterium- or plant-only treatment. Inoculation of Acinetobacter sp. S-33 increased the growth of both roots and shoots by more than 40%, and positively influenced the soil microflora. We conclude that the new oil-degrading strains, Acinetobacter sp. S-33 and A. marplatensis 101n, can serve as the basis for new bioremediation agents for the treatment of oil contaminated soils and waste.