Screening and molecular identification of Cr(VI)-resistant Trichoderma isolated from ex-tin mining soil in Bangka Belitung Province, Indonesia

International Symposium on Bioremediation, Biomaterial, Revegetation, and Conservation

生物科学;自然科学（总论）

Hastuty, A.^1 ; Hidayat, I.^1

Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jl. Raya Jakarta-Bogor KM 46, Cibinong, West-Java

16911, Indonesia^1

关键词: Bioremediation agent;    Electroplating industry;    Hexavalent chromium;    Internal transcribed spacers;    Molecular identification;    Pharmaceutical industry;    Rivers and lakes;    Wastewater treatment plants;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/308/1/012011/pdf
DOI  :  10.1088/1755-1315/308/1/012011

来源: IOP

【 摘 要 】

Several major industries in Indonesia such as the textile industry, electroplating industry, and the pharmaceutical industry, can generate a considerable amount of chromium (Cr) wastes, hexavalent chromium [Cr(VI)]. Unfortunately, the wastewater treatment plant systems of these industries may be partially unqualified, causing heavy pollution in several water bodies such as rivers and lakes. The aim of the present study was to discover and identify Cr(VI)-resistant fungi as bioremediation agents of removing the Cr(VI) from the environment. Seven fungal isolates from tin mining soil of Bangka Belitung province were found tolerant to high Cr(VI) concentration. All fungal isolates grew well at 0.25 mM of Cr(VI), and five fungal isolates still tolerant at a concentration of 2 mM. Molecular identification based on the ITS (internal transcribed spacer) rDNA region showed that these isolates belong to Trichoderma crassum (2 isolates), T. virescentiflavum (3 isolates), and T. aff. tomentosum (2 isolates).