Characterization of Bacillus thuringiensis Berl. indigenous from soil and its potency as biological agents of Spodoptera litura (Lepidoptera: Noctuidae)

International Symposium on Food and Agro-biodiversity 2017

农业科学;生物科学

Pujiastuti, Y.^1 ; Astuti, D.T.^2 ; Afriyani, S.R.^2 ; Suparman, S.^1 ; Irsan, C.^1 ; Sembiring, E.R.^3 ; Nugraha, S.^3 ; Mulawarman^1 ; Damiri, N.^1

Department of Plant Protection, Faculty of Agriculture, Sriwijaya University, Jalan Padang Selasa, Palembang, Indonesia^1

Graduate School of Agriculture, Faculty of Agriculture, Sriwijaya University, Jalan Padang Selasa, Palembang, Indonesia^2

Genomic and Crop Improvement Laboratory, Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km. 46, Cibinong, Bogor, Indonesia^3

关键词: Bacillus thuringiensis;    Biological agents;    Crystal proteins;    entomopathogene;    pest;    Sds-page electrophoresis;    Spodoptera litura;    Thuringiensis;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/102/1/012064/pdf
DOI  :  10.1088/1755-1315/102/1/012064

来源: IOP

【 摘 要 】

The objective of the study was to characterize the molecular weight of protein in order to be utilized as biological agent of S. litura and their cell or spores production. To investigate the molecular weight of protein was done by SDS-Page electrophoresis. Growth media used for producing B. thuringiensis were T3, LB broth and agricultural waste. The results showed that the molecular weight of protein ranged from 37 to 140 kDa. In DLM and DLKK23 isolates were found ranging from 37 to 40 kDa and from 110 to 130 kDa, respectively. KJ3R5 and KJ3P1 isolates were obtained having three protein bands ranging from 43 to 45, 73 to 80 and 110 to 130 kDa and 45-50, 75-80 and 130-140 kDa, respectively. It was predicted that isolates B. thuringiensis were belonging to Cry IA, Cry IIA, Cry IVC and Cry15c. These crystal proteins were toxic to S. litura. There was no protein bands found in the two last isolates (KJ3R3 and KJ3J4). Production of spore after sporulation in agricultural waste media ranged from 0.5 to 106- 2.67 x 107spores/ml showing medium level of toxicity to S. litura.