This report describes the test events and numbers of samples comprising an experimental design developed to assess sampling strategies and methods for detecting contamination in a building and releasing the building for use after decontamination. Idaho National Laboratory (INL) identified Building PBF-632 as a test bed facility for evaluating protocols for response to potential contamination by biological agents. Building PBF-632 is an unoccupied, two-story office building with each floor having an area of 4025 feet. The first floor has 11 offices, a reception area, men's and women's restrooms, and a mechanical room. The second floor has 15 offices, two storage rooms, mens and womens restrooms, and a mechanical room. Building PBF-632 will be contaminated with BG (Bacillus globigii, subsequently Bacillus subtilis var. niger, and recently renamed Bacillus atrophaeus), a simulant for Bacillus anthracis (BA). The contamination, sampling, decontamination, and re-sampling will occur as specified by the experimental design. This study is referred to as the INL Sample Collection Operational Test. Two objectives were developed to guide the construction of the experimental design for the INL Sample Collection Operational Test.