Preliminary studies on immobilization of lipase using chicken eggshell

International Conference on Chemical and Bioprocess Engineering

地球科学;化学;生物科学

Salleh, S.^1 ; Serri, N.A.^1 ; Hena, S.^1 ; Tajarudin, H.A.^1

School of Industrial Technology, Universiti Sains Malaysia, 11800, Malaysia^1

关键词: Candida antarctica;    Candida rugosa;    Crude enzymes;    Incubation time;    Lipase immobilization;    Mineral component;    Organic components;    Sugar esters;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/36/1/012026/pdf
DOI  :  10.1088/1755-1315/36/1/012026

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

A few advantages of enzyme immobilization are reusability of expensive enzyme, improvement of stability and activity compared to crude enzyme. Various organic components can be used as carrier for enzyme immobilization such as chicken eggshell. It can be used as a carrier for immobilization as its mineral component mostly contains of calcium carbonate. In the present study, Tributyrin method was used to test enzyme activity of Rhizomucour Miehei, Candida Antarctica and Candida Rugosa. Rhizomucour Miehei shows the highest enzyme activity (360.8 mol/min/mL lipase) and was used in further experiment. Experiment was continued to study incubation time for lipase immobilization on eggshell (1-4 hours) and reaction time of esterification of sugar ester (0-72 hours). Two hours incubation time for lipase immobilization was observed and gives the highest yield of sugar ester (78.13%). Fructose and stearic acid as substrate was used for the production of sugar ester. The highest percentage of sugar ester production was shown at 36 hours of reaction time.