A study on antioxidative and antimicrobial activities of saliva extract of Indonesian local leeches

2nd International Conference on Natural Products and Bioresource Sciences - 2018

生物科学;生态环境科学

Malik, B.^1^2 ; Astuti, D.A.^3 ; Arief, D.J.F.^3 ; Rahminiwati, M.^4

Bogor Agricultural University, Bogor, Indonesia^1

Faculty of Agriculture, Djuanda University, Bogor, Indonesia^2

Faculty of Animal Science, Bogor Agricultural University, Bogor, Indonesia^3

Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia^4

关键词: Anti-microbial activity;    Antioxidative;    Bacterial growth;    Completely randomized designs;    Minimum inhibitory concentration;    Total protein contents;    West sumatra;    Zone of inhibitions;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/251/1/012061/pdf
DOI  :  10.1088/1755-1315/251/1/012061

来源: IOP

【 摘 要 】

Antioxidative and antimicrobial activities of saliva extract of Indonesian leeches were assessed. Leeches were obtained from Situgede Bogor, Sawangan Depok, and Sijunjung Regency, West Sumatra. A completely randomized design with three treatments and five replicates was used. Treatments consisted of three places of leech origins. Measurements were taken on the amount, total protein content, IC50 value, zone of inhibition to bacterial growth, and minimum inhibitory concentration of crude leech saliva extract (CLSE). Results showed that the amount of CLSE obtained was 3.696-4.633g/g BW and total protein content of CLSE was 48.303-64.742 μg/mg. The IC50 value was 47-108 μg/mg while that of ascorbic acid was 3.312 μg/mg. Antimicrobial activity was found to the growth of S. aureus but not to the growth of E. coli with a minimum inhibitory concentration at 2.472-4.528 μg/ml.