Evaluation of antibacterial activity of three endodontic sealers against three bacterial strains isolated from root canal. An in vitro study

International Conference on Materials Engineering and Science

Mohammed, Haitham Younis^1 ; Younis, Hadeel Mizher^2 ; Khalaf, Hiba Younis^2

College of Dentistry, Tikrit University, Iraq^1

College of Dentistry, Tikrit University, Medical Microbiology, Iraq^2

关键词: Anti-bacterial activity;    Anti-microbial activity;    Antibacterial action;    Bacterial growth;    Bacterial strains;    Control materials;    Inhibitory effect;    Staphylococcus aureus;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/454/1/012171/pdf
DOI  :  10.1088/1757-899X/454/1/012171

来源: IOP

【 摘 要 】

This study evaluated in vitro antibacterial action of three endodontic sealer (zinc oxide Zn, carbon hydroxide CaOH and polyoxymethylene Dexamethason PD) against three bacterial strains commonly found in root canal infection which includes Serratia merasences, Enterococcus feacalis, and Staphylococcus aureus by using agar diffusion method on muller hinton agar and distilled water used as control material. Thirty petriplates(10 petriplates for Serratia merasences, 10 petriplates for Enterococcus feacalis, and 10 petriplates for Staphylococcus aureus ) with 25ml of muller hinton agar were inoculated with 0.1 ml of tested suspensions of tested bacteria(Serratia merasences, Enterococcus feacalis, and Staphylococcus aureus). Four cavities each one measuring 5ml in diameter and 4 ml in depth, were made in each muller hinton agar plates using cork poorer and then it filled completely with experimental product. After incubation at 37c for 24hrs the zone of bacterial growth inhibition produced around the well was measured in mm in diameter. In this study carbon hydroxide proved to be the most effective sealer against three tested bacteria Serratia merasences, Enterococcus feacalis, and Staphylococcus aureus, this is followed by PD sealer which also showed antimicrobial activity against the three tested microorganisms higher than zinc oxide which showed the least action on three tested bacteria. In Conclusion : All the three endodontic sealers evaluated in this study showed different inhibitory effect against the three tested bacterial strains (Serratia merasences, Enterococcus feacalis, and Staphylococcus aureus)