The effect of cytoglobin gene inhibition on fibroblast keloid cells proliferation

12th Congress of Indonesian Soc. for Biochemistry and Molecular Biology in Conjunction With The 2nd Int. Conf. "Collaboration Seminar of Chemistry and Industry

生物科学;化学

Jusman, S.W.A.^1^2 ; Siregar, F.M.^3^4 ; Sadikin, M.^1^2 ; Hardiany, N.S.^1^2

Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia^1

Center of Hypoxia and Oxidative Stress Studies, Jakarta, Indonesia^2

Master Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6, Jakarta, Indonesia^3

Department of Biochemistry, Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia^4

关键词: Cytoglobin;    Expression levels;    MTS assays;    Q-RT-PCR;    Tumor suppressor genes;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/217/1/012038/pdf
DOI  :  10.1088/1755-1315/217/1/012038

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

Cytoglobin (Cygb) is a new protein from the globin family whose function is reported to be either a tumor suppressor gene or oncogene. The aim of this study was to determine the function of Cygb on fibroblast keloid cells proliferation. The relative expressions of Cygb were compared between the siRNA (+) Cygb and siRNA (-) groups using qRT PCR, and their effect on cells proliferation, which was determined using the MTS assay. The results showed that the expression level of Cygb on siRNA (+) Cygb group were decreased compared to siRNA (-) group (0.315 vs 1.056; p = 0.000). However at the level of cell proliferation, there were no significant differences between the two groups (1.489 vs 1.359; p = 0.087). Based on the results, it is concluded that Cygb has no effect on fibroblast keloid cells proliferation.