Chitosan blend iron oxide nanostructure-based biosensor for healthy & malignant tissue glucose/urea detection

7th Global Conference on Materials Science and Engineering

工业技术（总论）;材料科学

Akram, M.W.^1^2 ; Alam, M.F.^1^3^4 ; Ji, H.N.^1^2 ; Mahmood, A.^3 ; Munir, Tariq^3 ; Iqbal, M.Z.^4 ; Saleem, M.R.^3 ; Amin, N.^3 ; Wu, A.G.^4

Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology, Sichuan province, Chengdu, China^1

School of Materials and Energy, University of Electronic Science and Technology of China, Chengdu

610054, China^2

Department of Physics, GC University, Faisalabad

38000, Pakistan^3

Key Laboratory of Magnetic Materials and Devices, Division of Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, No. 1219 Zhongguan West Road, Ningbo, China^4

关键词: Active surface area;    Aqueous chemical growth;    Chitosan composites;    Controlled morphology;    Electron transfer;    Malignant tissues;    Oxide nanostructures;    Potentiometric response;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/474/1/012060/pdf
DOI  :  10.1088/1757-899X/474/1/012060

来源: IOP

【 摘 要 】

A urease trapped chitosan-Fe3O4 based nano-composite biosensor of controlled morphology is successfully fabricated by applying the aqueous chemical growth technique. Final product of nanodevice was characterized by applying various techniques. Log of the concentration of various solutions (1 to 80 mM) ranged between 0.0 to 2.5 mM for urea and glucose sample and an output signal of ∼ 42 mV per decade was detected at room temperature. It disclosed the presence of chitosan composite with Fe3O4 results enhanced active surface area of sensing along with up to the mark precession, as compared to traditional biosensor for immobilization of enzymes. The current developed form of chitosan-Fe3O4 nano-composite biosensor illustrated a significant stable potentiometric response in the minimal time period. Besides, nafion/polypyrrole addition to the top surface of CH-Fe3O4 nanocomposite not only protected it from degradation but also upgraded the characteristic of sensor regarding rapid electron transfer enhancement and higher shelf life of this hybrid form of nanomaterial. The advanced form of this voltammetry implied that Ur-GLDH/CH-Fe3O4 bioelectrode is found to be very sensitive in the range of 4-120 mg/dl urea/glucose concentration and can detect as low amount as 0.4mg/dl.