This report highlights the value of flow cytometry analysis, particularly in the setting of myeloproliferative neoplasms showing features of progression, as neoplastic plasmacytoid dendritic cell (PDC) proliferations may be present, representing either a clonal expansion of mature PDCs related to the underlying myeloproliferative neoplasm or transformation to blastic plasmacytoid dendritic cell neoplasm (BPDCN). BPDCN should always be considered in patients with myeloid neoplasms in progression and/or who develop new cutaneous findings, as it may prompt change of management.

A 48-year-old woman was diagnosed with B-acute lymphoblasticleukemia (B-ALL) in 1996 and was treated with chemotherapy andan allogeneic sex mismatched unrelated donor stem cell transplant(SCT) in 1997 at another institution. She presented to our hospital in January 2020 with pancytopenia. Bone marrow (BM) biopsy(Figure 1) showed hypercellular (95%) BM with sheet of blasts (A,hematoxylin and eosin, 10×). Aspirate smears showed 80% blaststhat were large with dispersed chromatin, inconspicuous nucleoli, andbasophilic cytoplasm with numerous vacuoles reminiscent of Burkittlike morphology (B, Wright and Giemsa, 50×). Flow cytometry (FC)immunophenotyping showed B-lymphoblasts positive for CD19, CD20(subset), CD22, CD34, CD38 (decreased), CD79a, HLA-DR, and TdT (Cand D) with an aberrant myeloperoxidase (MPO) expression (E) thatwas dimmer in blasts (red) compared with background granulocytes(gray). Blasts were negative for myeloid and/or monocytic markersincluding CD11c, CD13, CD14, CD33, CD64, CD117, and lysozyme.Immunohistochemistry highlighted blasts positive for Pax5 (F, 20×) andMPO with a granular cytoplasmic staining pattern (G, 20×). Cytochemistry showed 35% of blasts positive for MPO (H, 500×) and negativefor butyrate esterase.

Epithelial-to-mesenchymal transition (EMT) plays a significant role in cancer metastasis. A series of models have focused on EMT regulation by TGF-β network. However, how EMT is regulated under hypoxia is less understood. We developed a model of HIF-1α network to explore the potential link between EMT and the network topology. Our results revealed that three positive feedback loops, composed of HIF-1α and its three targets SNAIL, TWIST, and miR-210, should be sequentially activated to induce EMT under aggravating hypoxia. We suggested that the number of the positive feedback loops is critical for determining the number of stable states in EMT. Our work may advance the understanding of the significance of network topology in the regulation of EMT.

Volatile organic compounds are a kind of important indoor and outdoor air pollutants. In recent years, more and more attention has been paid to the ways of volatile organic compound elimination because of its potential long-term effects on human health. Among the various available methods for volatile organic compound elimination, the catalytic combustion is the most attractive method due to its high efficiency, low cost, simple operation, and easy scale-up. Perovskite oxides, as a large family of metal oxides with their A-site mainly of lanthanide element and/or alkaline earth metal element and B-site of transition metal element, have been extensively investigated as active and stable catalysts for volatile organic compound removal reactions due to their abundant compositional elements, high thermal/chemical stability, and compositional/structural flexibility. The catalytic performance of perovskite oxides is strongly depended on its material composition, morphology, and surface/bulk properties, while the doping, tailored synthesis route, and composite construction may have a significant effect on the bulk (oxygen vacancy concentration, lattice structure), surface (oxygen species, defect) properties, and particulate morphology, consequently the catalytic activity and stability for volatile organic compound removal. Herein, a comprehensive review about the recent advances in perovskite oxides for volatile organic compound elimination reactions based on catalytic combustion is presented from different aspects with a special emphasis on the material design strategies, such as compositional tuning, morphology control, nanostructure building, hybrid construction, and surface modification. At last, some perspectives are presented on the development and design of perovskite oxide-based catalysts for volatile organic compound removal applications by highlighgting the critical issues and challenges.