Iberin inhibits adipogenesis in 3T3-L1 preadipocytes by suppressing Akt signaling

Iberin;Akt signaling;adipogenesis;3T3-L1 preadipocytes;571

농업생명과학대학 농생명공학부(바이오모듈레이션전공) ;

University:서울대학교 대학원

关键词: Iberin;    Akt signaling;    adipogenesis;    3T3-L1 preadipocytes;    571;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/126044/1/000000012627.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

Iberin is a naturally derived isothiocyanate in cruciferous vegetables such as savoy cabbage, cauliflower, and broccoli. It has been reported to exhibit anti-cancer effects. However, anti-obesity effect and its molecular mechanism have not been elucidated. Herein, I examined the anti-adipogenic effect of iberin and its associated mechanisms in 3T3-L1 preadipocytes. Iberin effectively inhibited MDI (Methyl isobutylxanthine, Dexamethasone, Insulin)-induced adipogenesis in the early stage of adipocyte differentiation and the expression of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα) which are key regulators of adipogenesis and lipid accumulation. Fluorescein-activated cell sorter (FACS) analysis data showed that iberin suppressed MDI-induced cell cycle progression, mitotic clonal expansion (MCE). Western blotanalysis demonstrated that not extracellular-signal-regulated kinase (ERK) but Akt signaling was attenuated by iberin. Collectively, these finding suggest thatiberin possesses anti-adipogenic activity by suppressing Akt signaling pathway.