Isolation of Phenolic Compounds from Arecae Pericarpium Guided by Inhibition of LPS-Induced Nitric Oxide Production in RAW 264.7 Cells

Arecae Pericarpium;Areca catechu (L.);HPCCC;phenols;bioassay-guided isolation;nitric oxide inhibition;615

약학대학 약학과 ;

University:서울대학교 대학원

关键词: Arecae Pericarpium;    Areca catechu (L.);    HPCCC;    phenols;    bioassay-guided isolation;    nitric oxide inhibition;    615;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/133589/1/000000053312.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

Isolation of Phenolic Compounds from Arecae Pericarpium Guided by Inhibition of LPS-Induced Nitric Oxide Production in RAW 264.7 CellsRAW 264.7 세포내 지질다당류로 유도된 산화질소의 생성 억제에 따른 대복피 유래 페놀성 화합물의 분리August 2015 Amelia IndrianaNatural Product Science MajorCollege of PharmacyMaster Course in Graduate SchoolSeoul National University AbstractIsolation of Phenolic Compounds from Arecae Pericarpium Guided by Inhibition of LPS-Induced Nitric Oxide Production in RAW 264.7 CellsAmelia IndrianaNatural Products Science MajorCollege of PharmacyMaster Course in the Graduate SchoolSeoul National UniversityArecae Pericarpium is the nut husk of Areca catechu L. (Arecaceae). In Traditional Chinese Medicine, it is used in Huo Xiang Zheng Qi formula to cure abdominal distension, vomiting, and diarrhea and Gami-Jeonggisan formula in Traditional Korean Medicine to treat vascular diseases. Arecae Pericarpium has been studied for its antioxidative activity, antifungal activity, and phytochemical compounds isolation. However, its antiinflammatory activity has not been reported yet. This study aimed to isolate compounds from Arecae Pericarpium guided by the inhibition of nitric oxide production on RAW 264.7 macrophage cells.Dried Arecae Pericarpium was macerated in methanol. The macerate was evaporated, dissolved in 10% methanol and partitioned with hexane, methylene chloride, ethyl acetae and n-butanol. Methylene chloride and ethyl acetate fraction, which inhibited nitric oxide production to lower levels compared to other fractions, were further separated. Methylene chloride fraction was subjected to repeated silica gel column chromatography to obtain seven fractions (MC1A to MC1G fraction). MC1A, MC1B and MC1G fractions inhibited the LPS-induced nitric oxide production. MC1G was obtained as single compound and identified as syringol dimer (compound I). MC1A was subjected through Diaion® HP-20 column chromatography and catechol (compound II) was obtained from 20% and 30% MeOH column fraction. Four compounds, 4-hydroxybenzaldehyde (compound III), vanillin (compound IV), 4-hydroxyacetophenone (compound V) and apocynin (compound VI), were isolated from MC1B fraction by combining HPCCC (hexane-ethyl acetate-methanol-water system 2:5:1:4 v/v) and preparative HPLC. Ethyl acetate fraction was subjected to Diaion® HP-20 column chromatography and 20% MeOH column fraction was separated using high performance counter-current chromatography (hexane-ethyl acetate-methanol- water system 2:5:2:5 v/v) to obtain protocatechuic acid (compound VII) and 4-hydroxybenzoic acid (compound VIII). All isolated compounds were examined and the result suggested that catechol might be the primary contributing active compound to the suppression of LPS-induced NO production by Arecae Pericarpium.