Development of a cell-laden thermosensitive chitosan bioink for 3D bioprinting

3D printing;Bioprinting;bioink;hydrogel;chtiosan;660.6

농업생명과학대학 바이오시스템·소재학부 ;

University:서울대학교 대학원

关键词: 3D printing;    Bioprinting;    bioink;    hydrogel;    chtiosan;    660.6;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/141780/1/000000149945.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

3D bioprinting is a technology used to deposit cell-laden biomaterials for the construction of complex tissue. For 3D bioprinting of cell-laden construct, many materials have been developed such as alginate, fibrin and gelatin. However, most of these hydrogels are chemically bound using crosslinkers which can cause some problems in cytotoxicity and cell viability. On the other hand, thermosensitive hydrogels are physically cross-linked by non-covalent interaction without crosslinker, facilitating stable cytotoxicity and cell viability. The typical examples of thermosensitive hydrogels are methyl cellulose, hydroxypropyl cellulose, N-isopropylacrylamide(NiPAAM), poly(ethylene glycol)/poly(propylene glycol)/poly(ethylene glycol) (PEG-PPG-PEG) and poly(ethylene glycol)/poly(lactic acid-co-glycolic acid) (PEG/PLGA). Chitosan, which has non-toxic, biocompatible and biodegradable polysaccharide composed of glucosamine and derived by deacetylation of chitin, can be used as thermosensitive hydrogels. In chitosan thermosensitive hydrogels, gelling agent and solvent are the key factors for sol-gel transition. Yet, there is no study to investigate the comparison among these gelling agents or solvent for bioink. Therefore, the purpose of this study was to develop and compare bioinks by chitosan hydrogels for 3D bioprinting. Solvent did not affect the gel shape and gelation time. However, acetic acid had good biocompatibility compared with lactic acid and hydrochloric acid. Gelling agent was more important factor than solvent. Gel shape and gelation time were different among gelling agent. NaHCO3 had greater growth rate of the storage modulus (G′) and more irregular porous structure than β-GP and K2HPO4. From the water soluble tetrazolium salt (WST) and live and dead assay data, it was proven NaHCO3 had good effect for cell adhesion. In cell-laden construct, there was no big difference among gelling agent. However, NaHCO3 had good printability compared with β-GP and K2HPO4.