Applications of functional polymer brushes for nanoparticle uptake and preventionof protein adsorption

Polymer brush;Protein adsorption;Nano-particle adsorption

Arifuzzaman, Shafi Mahmud ; Dr. Jan Genzer, Committee Member,Dr. Saad Khan, Committee Member,Dr. Orlin D. Velev, Committee Member,Dr. Orlando Rojas, Committee Member,Arifuzzaman, Shafi Mahmud ; Dr. Jan Genzer ; Committee Member ; Dr. Saad Khan ; Committee Member ; Dr. Orlin D. Velev ; Committee Member ; Dr. Orlando Rojas ; Committee Member

University:North Carolina State University

关键词: Polymer brush;    Protein adsorption;    Nano-particle adsorption;   

Others  :  https://repository.lib.ncsu.edu/bitstream/handle/1840.16/6154/etd.pdf?sequence=1&isAllowed=y

美国|英语

来源: null

【 摘 要 】

The central theme of this Ph.D. dissertation is to develop novel multifunctionalpolymer coatings for understanding partition of proteins and nanoparticles on polymersgrafted to flat surfaces (so-called brushes). Systematic investigation of the adsorptionphenomena is accomplished by utilizing surface-anchored assemblies comprising graftedpolymers with variation in physical properties (i.e., length or/and grafting density) andchemical functionality. The chemical composition of the brush is tailored by either“chemical coloringâ€of a parent homopolymer brush with selective chemical moieties or bysequential growth of two chemically dissimilar polymer blocks. We present preparation oftwo types of tailor-made, surface-grafted copolymers: 1) those composed of hydrophilic andhydrophobic blocks (so-called amphiphilic polymer brushes), and 2) those comprising ofanionic and cationic polymer segments (so-called polyampholyte brushes). We describe theorganization of functionality in the grafted polymer brushes and the partitioning of proteinsand nanoparticles using a battery of complementary analytical probes. Specifically, weaddress how varying the molecular weight, grafting density, and chemical composition of thebrush affects adsorbtion and desorbtion of model proteins and gold nanoparticles. Ourobservations indicate densely-populated responsive amphiphilic polymers are very efficientin suppressing protein adsorption. In addition, we have established that the length of poly(ethylene glycol) spacers attached to a parent homopolymer brush is a key factorgoverning uptake of gold nanoparticles. Both grafting density and molecular weight of thecoating are important in controlling the kinetics and thermodynamics of protein adsorptionon surfaces. Our findings and methodologies can lead to the development of next generationenvironmentally friendly antifouling surfaces and will find application in medical devices,antifouling coatings and anti reflection finishes.