Correlation Study of PVDF Membrane Morphology with Protein Adsorption: Quantitative Analysis by FTIR/ATR Technique

3rd International Conference on Global Sustainability and Chemical Engineering

工业技术;化学工业

Ideris, N.^1 ; Ahmad, A.L.^2 ; Ooi, B.S.^2 ; Low, S.C.^2

Faculty of Chemical Engineering, Universiti Teknologi MARA, Selangor, Shah Alam

40450, Malaysia^1

School of Chemical Engineering, Universiti Sains Malaysia, Nibong Tebal, Pulau Pinang

14300, Malaysia^2

关键词: Colour change;    Correlation coefficient;    Correlation studies;    Membrane pore size;    Protein adsorption;    Protein concentrations;    PVDF membrane;    Surface proteins;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/358/1/012054/pdf
DOI  :  10.1088/1757-899X/358/1/012054

学科分类：工业工程学

来源: IOP

【 摘 要 】

Microporous PVDF membranes were used as protein capture matrices in immunoassays. Because the most common labels in immunoassays were detected based on the colour change, an understanding of how protein concentration varies on different PVDF surfaces was needed. Herein, the correlation between the membrane pore size and protein adsorption was systematically investigated. Five different PVDF membrane morphologies were prepared and FTIR/ATR was employed to accurately quantify the surface protein concentration on membranes with small pore sizes. SigmaPlot® was used to find a suitable curve fit for protein adsorption and membrane pore size, with a high correlation coefficient, R2, of 0.9971.