Comparative Analysis of RNase P

holoenzyme;comparative analysis;RNase P

Gantt, John Arthur ; Dr. Amy M. Grunden, Committee Member,Dr. Stephen J. Libby, Committee Member,Dr. James W. Brown, Committee Chair,Gantt, John Arthur ; Dr. Amy M. Grunden ; Committee Member ; Dr. Stephen J. Libby ; Committee Member ; Dr. James W. Brown ; Committee Chair

University:North Carolina State University

关键词: holoenzyme;    comparative analysis;    RNase P;   

Others  :  https://repository.lib.ncsu.edu/bitstream/handle/1840.16/2555/etd.pdf?sequence=1&isAllowed=y

美国|英语

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【 摘 要 】

This Master's Thesis contains a description of the results of two research projects that were worked on over the course of two years.The first was an attempt to heterologously reconstitute the Bacillus subtilis RNase P RNA with the RNase P protein subunits of Methanothermobacter thermoautotrophicus, to identify a functional homology between one or more of the archaeal proteins with the Bacillus subtilis RNase P protein. Unfortunately, the reconstitution experiment could not be completed due to the poor quality of the pre-tRNAasp substrate synthesized.The second project dealt with comparative analysis of bacterial RNase P RNA.Eleven new RNase P RNA genes were amplified, sequenced, and their secondary structures derived by comparative analysis.