Structural Biology of Bacterial RNA Polymerase

Biomolecules

Katsuhiko S. Murakami¹  Sivaramesh Wigneshweraraj¹ 

[1] Department of Biochemistry and Molecular Biology, The Center for RNA Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA; E-Mail

关键词: bacterial RNA polymerase;    transcription;    core enzyme;    holoenzyme;    σ factor;    transcription factor;    anti-σ factor;    X-ray crystallography;    NMR;    inhibitor antibiotic;   

DOI  :  10.3390/biom5020848

来源: mdpi

【 摘 要 】

Since its discovery and characterization in the early 1960s (Hurwitz, J. The discovery of RNA polymerase. J. Biol. Chem. 2005, 280, 42477–42485), an enormous amount of biochemical, biophysical and genetic data has been collected on bacterial RNA polymerase (RNAP). In the late 1990s, structural information pertaining to bacterial RNAP has emerged that provided unprecedented insights into the function and mechanism of RNA transcription. In this review, I list all structures related to bacterial RNAP (as determined by X-ray crystallography and NMR methods available from the Protein Data Bank), describe their contributions to bacterial transcription research and discuss the role that small molecules play in inhibiting bacterial RNA transcription.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.