A Simplified Visible/Near-Infrared Spectrophotometric Approach to Blood Typing for Automated Transfusion Safety

LED;spectrophotometric;spectroscopic;blood typing;IRED

Anthony, Steven R ; Dr. Ashok Gopalarathnam, Committee Member,Dr. Kara J. Peters, Committee Member,Dr. M.K. Ramasubramanian, Committee Chair,Anthony, Steven R ; Dr. Ashok Gopalarathnam ; Committee Member ; Dr. Kara J. Peters ; Committee Member ; Dr. M.K. Ramasubramanian ; Committee Chair

University:North Carolina State University

关键词: LED;    spectrophotometric;    spectroscopic;    blood typing;    IRED;   

Others  :  https://repository.lib.ncsu.edu/bitstream/handle/1840.16/1849/etd.pdf?sequence=1&isAllowed=y

美国|英语

来源: null

【 摘 要 】

A new technique has recently been introduced for objectively quantifying the agglutination of red blood cells in a blood typing procedure using ultraviolet/visible spectroscopy.The technique involves analyzing the spectra of red blood cell suspensions in saline between the 665 nm and 1000 nm range, where the relative slope between a control and antibody treated sample are entered into a simple algorithm to form a so called agglutination index (A.I.).The proposal of this research is to simplify the detection method by replacing the spectral imaging of the diode array spectrophotometer with a discrete series of LED and photodiode pairs within the wavelength range of interest, in the forward scattering direction.The scattering theory involved in this phenomenon is investigated, and a simplified experimental sensor is designed and evaluated.The resulting experimentation shows a significant recreation of the spectrophotometer results by the simplified design with a promising potential for improvement.Optoelectronic design considerations are discussed for maximizing the sensitivity of this technique for use in a cost effective, automated device for transfusion safety.