De-differentiation of parotid acinar cells in vitro.

Epigenetics;Methylation;Salivary;Gland;De-differentiation;Parotid

Andrew Scott Currie, 1983-

University:University of Louisville

Department:Oral Biology

关键词: Epigenetics;    Methylation;    Salivary;    Gland;    De-differentiation;    Parotid;   

Others  :  https://ir.library.louisville.edu/cgi/viewcontent.cgi?article=1302&context=etd

美国|英语

来源: The Universite of Louisville's Institutional Repository

【 摘 要 】

Background: When rat salivary gland cells are cultured in vitro, they dedifferentiate within 24 h. Hypothesis: Growth factors will prevent de-differentiation of primary cultures of parotid gland cells, and they will induce differentiation of ParC5 cells. DNA methylation drives de-differentiation and the loss of expression in cultured parotid cells. Methods: Rat parotid glands were collected and cultured in media using different combinations of growth factors. DNA was evaluated for methylation at various time points. Results: No media tested prevented the de-differentiation of parotid cells, and none induced differentiation of ParC5 cells. Limited variation in methylation of CpG sites was seen. Conclusion: Growth factors do not prevent de-differentiation in primary cell culture, nor do they activate differentiation in ParC5 cells. Global methylation of Mist1 does not cause de-differentiation; evidence supports that methylation of PSP increases over time.