Quantitative phase imaging for blood cytometry

Quantitative Phase Imaging;Blood Cytometry;Interferometry;Single Cell Morphology

Mir, Mustafa A. ; Popescu ; Gabriel

关键词: Quantitative Phase Imaging;    Blood Cytometry;    Interferometry;    Single Cell Morphology;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/18321/Mir_Mustafa.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Blood smear analysis has remained a crucial diagnostic tool for pathologists despite theadvent of automatic analyzers such as flow cytometers and impedance counters. Though thesecurrent methods have proven to be indispensable tools for physicians and researchers alike, theyprovide limited information on the detailed morphology of individual cells and merely alert theoperator to manually examine a blood smear by raising flags when abnormalities are detected.Here I demonstrate an automatic interferometry based smear analysis technique known asDiffraction Phase Cytometry (DPC), which is capable of providing the same information on redblood cells as is provided by current clinical analyzers, while rendering additional, currentlyunavailable parameters on the 2D and 3D morphology of individual red blood cells (RBCs). Tovalidate the utility of the technique in a clinical setting, I present a comparison between testsgenerated from 32 patients by a state-of-the-art clinical impedance counter and DPC.The majority of work presented in this thesis is drawn from two publications: M. Mir etal., Journal of Biomedical Optics, vol. 15 (2), 2010, and M. Mir et al. Optics Express, vol. 17,2009.