Cell migration and alignment on mechanically controlled environment

cell migration;cell alignment;contact guidance;cell-matrix interaction

Kwon, Gubeum

关键词: cell migration;    cell alignment;    contact guidance;    cell-matrix interaction;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/78560/KWON-THESIS-2015.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

This thesis studies the effect of mechanically controlled environment on cell migration and alignment in vitro. Understanding such behaviors are necessary to study wound healing of life, metastatic of cancer cells, tissue engineering, and regenerative medicines. First study is a cell migration behavior on stiffness controlled three-dimensional in vitro environment. The continuous stiffness gradient 3D environment is provided by the effective stiffness on a PDMS wavy pattern, and the relationship between stiffness gradient and the cell migration is analyzed. Next, the effect of cell-cell interaction on cell alignment on a PDMS wavy pattern is studied. The previous study about the cell alignment behavior with feature characteristics is repeated for further research. Lastly, we look at the cell development behavior on various micro scale structures. Tubule etching structures, ribbon-like 3D structures, and table structures are explored to observe the cell growth, migration, alignment, and even the effect of cells on those structures. Studying the mechanically controlled environment on cells would provide easier and more precise controllable methods for tissues engineering and regenerative medicine.