Optical micromanipulation using ultrashort pulsed laser sources

Laser manipulation (Nuclear physics);Micrurgy;Laser pulses, Ultrashort

Little, Helen ; Dholakia, Kishan ; Dholakia, Kishan

University:University of St Andrews

Department:Physics & Astronomy (School of)

关键词: Laser manipulation (Nuclear physics);    Micrurgy;    Laser pulses, Ultrashort;   

Others  :  https://research-repository.st-andrews.ac.uk/bitstream/handle/10023/338/thesis.pdf?sequence=1&isAllowed=y

来源: DR-NTU

【 摘 要 】

In this thesis two previously separate fields of study are brought together: optical micromanipulation and ultrashort laser research. Here, the benefits ofcombining the high peak powers of ultrashort pulsed lasers and conventionaloptical micromanipulation techniques are explored. As optical trapping hasbeen studied extensively, the focus of this research is on optical guiding. Moreover, the emphasis is on the use of Bessel beams as these have been shown to offer greater guiding distances than comparable Gaussian beams. The studies within this thesis show that optical guiding in Bessel and Gaussian beams is governed by the average power of the laser. However, the benefits of guiding with ultrashort pulsed lasers to exploit multi-photon processes become evident as the demonstration of simultaneous optical guiding and second harmonic generation in microscopic nonlinear crystal fragments is detailed. This work is developed by using ultrashort pulses to induce two-photon excitation-induced fluorescence in the guiding medium. This allows direct visualisation of the beam-particle interaction and measurement of the reconstruction of the Bessel beam around an object. Some studies using two-photon excitation to investigate Bessel beam penetration through turbid media are discussed. Finally, the work is concluded by exploring the use of pulsed white-light lasers in optical guiding. The wavelength-dependent propagation and reconstruction properties of the white-light Bessel beam are studied before some preliminary optical guiding experiments are discussed. From this, the broad bandwidth of the supercontinuum source is found to offer extended guiding distances in Gaussian beams thereby negating the need for Bessel beams.