Pancreatic Progenitor Cells in Mice

pancreas;ascl2;lhx1;progenitor cells;Human Genetics and Molecular Biology

Cleveland, Megan HusseyAndrew, Debra ;

Johns Hopkins University

关键词: pancreas;    ascl2;    lhx1;    progenitor cells;    Human Genetics and Molecular Biology;   

Others  :  https://jscholarship.library.jhu.edu/bitstream/handle/1774.2/60305/CLEVELAND-DISSERTATION-2014.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: JOHNS HOPKINS DSpace Repository

【 摘 要 】

We generated a novel transgenic mouse expressing a tdTomato fluorophore, as well as a Strep/Flag-tagged version of Ptf1a from the native Ptf1a locus.I crossed this mouse line with the well-characterized Pdx1-GFP mouse, which enabled us to visualize and sort for cells of the ;;tip” and ;;trunk” progenitor domains of the mouse pancreas.The main goal of our project was to identify previously unrecognized early transcriptional targets of Ptf1at and Pdx1. I isolated early E11.5 epithelial progenitors as well as later E13.5 tip and trunk progenitors using FACS and analyzed these populations via the GeneChip Mouse Gene 1.0 ST Array.After comparison of microarray data between tip and trunk cells, differentially-expressed genes were identified, with a focus on transcription factors, with validation by in situ hybridization.Two transcription factors, Ascl2 and Lhx1, were initially identified that had no previously known role in pancreas development, and were shown by ISH to be expressed in the expected domain of the pancreas.I performed prelimary functional studies on these two transcription factors, using lentiviral shRNAs for knock down in dorsal pancreatic bud culture.