Attempts to analyze the RNA-binding specificity of the C. elegans RNP-8 protein

Biochemistry;Biology

Wilson, TracyKimble, Judith (Mentor) ;

University of Wisconsin

关键词: Biochemistry;    Biology;   

Others  :  https://minds.wisconsin.edu/bitstream/handle/1793/46048/2010_Wilson.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: University of Wisconsin

【 摘 要 】

Cytoplasmic polyadenylation is a key mechanism of gene regulation in all eukaryotes. The C. elegans GLD-2 protein provides the catalytic activity for a cytoplasmic poly(A) polymerase, but GLD-2 must partner with another protein that provides RNA-binding activity to produce a fully functional enzyme. One such GLD-2 binding partner is RNP-8, which contains an RNA recognition motif. Here, we explored the RNA-binding specificity of RNP-8. Using the yeast-three hybrid system, we identified 34 unique RNA sequences that were bound by RNP-8, but we could not identify a consensus sequence among the 34 sequences. Therefore, RNP-I protein may lack sequence specificity when binding to RNA.