Evaluation of copper alloy surfaces for inactivation of Tulane virus, a human norovirus surrogate, and human noroviruses.

Microbiology;Norovirus

Recker, JordanLi, Xinhui ;

University of Wisconsin

关键词: Microbiology;    Norovirus;   

Others  :  https://minds.wisconsin.edu/bitstream/handle/1793/78859/Recker_Jordan_Thesis.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: University of Wisconsin

【 摘 要 】

This study evaluated the efficacy of copper alloy surfaces for inactivation of Tulanevirus, a human norovirus surrogate, using plaque assay and porcine gastric mucin-conjugated magnetic beads (PGM-MB) binding assay followed by quantitative reversetranscription-PCR (PGM-MB/PCR assay). Additionally, the sensitivities of humannorovirus GII.4 Sydney and GI.3B Potsdam strains to copper alloy surfaces wereassessed using PGM-MB/PCR assay. Time-dependent inactivation of viruses on copperalloy coupons revealed that, for Tulane virus, 15 min of copper alloy surface treatmentsachieved more than 4-log reductions, as assessed by plaque assay, while up to 20 min ofcopper alloy surface treatments only achieved ~2-log reductions, as assessed by PGM-MB/PCR assay. As assessed by PGM-MB/PCR assay, 10 min of copper surfacetreatments achieved reductions of 3 and 4 log units for human norovirus GII.4 Sydneyand GI.3B Potsdam, respectively. Results from this study suggest that even though PGM-MB/PCR assay underestimated the efficacy of copper alloy surface inactivation of Tulanevirus, copper alloys could effectively inactivate Tulane virus and human noroviruses.Therefore, copper alloys can be used as a preventive measure to prevent human norovirusinfection and an effective surface treatment for human noroviruses.