Nanoscale Bio-Molecular Control Using EC-OWLS.

Bearinger, J. P. ; Voeroes, J. ; Hubbell, J. A. ; Textor, M.

Technical Information Center Oak Ridge Tennessee

关键词: Biomolecules;    Optical waveguides;    Control;    Adsorption;    Optical sensors;   

RP-ID  :  DE200415007475

学科分类：工程和技术（综合）

美国|英语

来源: National Technical Reports Library

【 摘 要 】

A recently developed technique termed 'Electrochemical Optical Waveguide Lightmode Spectroscopy' (EC-OWLS) combines evanescent-field optical sensing with electrochemical control of surface adsorption processes. Initial EC-OWLS investigations efficiently monitored molecular surface adsorption and layer thickness changes of an adsorbed polymer layer examined in situ as a function of potential applied to a waveguide1. A layer of indium tin oxide (ITO) served as both a high refractive index waveguide for optical sensing, and a conductive electrode; an electrochemical flow-through fluid cell incorporated working, reference and counter electrodes. Poly(L-lysine)- grafted-poly(ethylene glycol) (PLL-g-PEG) served as a model, polycation adsorbate. Results indicate that adsorption and desorption of PLL-g-PEG from aqueous buffer are a function of applied potential, and that binding events subsequent to PLL-g-PEG functionalization are dependent on reorganization in the molecular adlayer.