Characterization of propane monooxygenase: initial mechanistic studies.

Unkefer, P. ; Alvarez, M. ; Unkefer, C.

Technical Information Center Oak Ridge Tennessee

关键词: Contamination;    Enzymes;    Ground water;    Epoxides;    Hydrocarbons;   

RP-ID  :  DE2001773329

学科分类：工程和技术（综合）

美国|英语

来源: National Technical Reports Library

【 摘 要 】

Extensive industrial and DOE use of chlorinated hydrocarbons has resulted in widespread soil and ground-water contamination. Bioremediation is a potential remedy because various bacterial strains degrade chlorinated compounds, including trichloroethylene (TCE). Previous reports indicated that the propane monooxygenase (PMO) enzyme from Mycobacterium vaccae degraded TCE. These reports included additional substrates and resulting products that were inconsistent with PMO forming an epoxide intermediate; thus PMO appeared to be an attractive alternative to the epoxide-forming methane monooxygenase (MMO) enzyme. PMO had not been isolated and was largely uncharacterized. This project characterized PMO and its mechanism. PMO had a multi-component quaternary structure that was remarkably similar to that of MMO. The products formed by PMO from two key substrates were not as previously reported and were precisely those predicted by an epoxide intermediate. The observed lack of unique character of PMO has caused us to forgo further study.