Finding Regulatory Elements Using Joint Likelihoods for Sequence and Expression Profile Data.

Bruno, W. J. ; Holmes, I.

Technical Information Center Oak Ridge Tennessee

关键词: Genes;    Algorithms;    Internet;    Sampling;    Expression data;   

RP-ID  :  DE2001752619

学科分类：工程和技术（综合）

美国|英语

来源: National Technical Reports Library

【 摘 要 】

A recent, popular method of finding promoter sequences is to look for conserved motifs up-stream of genes clustered on the basis of expression data. This method presupposes that the clustering is correct. Theoretically, one should be better able to find promoter sequences and create more relevant gene clusters by taking a unified approach to these two problems. We present a likelihood function for a sequence-expression model giving a joint likelihood for a promoter sequence and its corresponding expression levels. An algorithm to estimate sequence-expression model parameters using Gibbs sampling and Expectation/Maximization is described. A program, called kimono, that implements this algorithm has been developed and the source code is freely available over the internet.