Effects of Simulated Microgravity on a Host-Pathogen System

Gilbert, Rachel ; Tran, Nhung ; Bhattacharya, Sharmila

关键词: SPACE ENVIRONMENT SIMULATION;    SPACE FLIGHT;    MICROGRAVITY;    BACTERIA;    MICROORGANISMS;    PATHOGENS;    ASTRONAUTS;    IMMUNE SYSTEMS;    INFECTIOUS DISEASES;    GENE EXPRESSION;    DROSOPHILA;    SERRATIA;    INTERNATIONAL SPACE STATION;    VIRULENCE;   

RP-ID  :  ARC-E-DAA-TN57604

学科分类：生物科学（综合）

美国|英语

来源: NASA Technical Reports Server

【 摘 要 】

While it has been shown that decades of astronauts and cosmonauts suffer from immune disorders both during and after spaceflight, the underlying causes are still poorly understood, due in part to the fact that there are so many variables to consider when investigating the human immune system in a complex environment. Invertebrates have become popular models for studying human disease because they are cheap, highly amenable to experimental manipulation, and have innate immune systems with a high genetic similarity to humans. Fruit flies (Drosophila melanogaster) have been shown to experience a dramatic shift in immune gene expression following spaceflight, but are still able to fight off infections when exposed to bacteria. Furthermore, a recent spaceflight mission showed that flies are more susceptible to infection following exposure to microgravity conditions, compared to ground-reared flies from the same population. Additionally, the common bacterial pathogen Serratia marcescens was shown to become more lethal to fruit flies (both space- and ground-reared) after being cultured in space, suggesting that not only do we need to consider host changes in susceptibility, but also changes in the pathogen itself after spaceflight conditions. Being able to simulate spaceflight conditions in a controlled environment on the ground gives us the ability to not only evaluate the effects of microgravity on the host immune system, but also how the microorganisms that cause immune disorders are being affected by these drastic environmental shifts. In this study, I use both spaceflight and ground-based (simulated microgravity) environments to examine the genetic changes associated with increased S. marcescens virulence in order to understand how microgravity is affecting this pathogen, as well as to evaluate how these genetic changes influence and interact with the host immune system. This study will provide us with more directed approaches to studying the effects of spaceflight on human beings, with the ultimate goal of being able to ameliorate human immune dysfunction in future space exploration.