Two Component Signal Transduction in Desulfovibrio Species

Luning, Eric ; Rajeev, Lara ; Ray, Jayashree ; Mukhopadhyay, Aindrila

Lawrence Berkeley National Laboratory

关键词: D. Vulgaris Hildenborough, Sulfate-Reducing Bacteria, Bioremediation;    In Vitro;    54;    Bioremediation;    Sulfate-Reducing Bacteria;   

DOI  :  10.2172/985938
RP-ID  :  LBNL-3760E-Poster
RP-ID  :  DE-AC02-05CH11231
RP-ID  :  985938

美国|英语

来源: UNT Digital Library

【 摘 要 】

The environmentally relevant Desulfovibrio species are sulfate-reducing bacteria that are of interest in the bioremediation of heavy metal contaminated water. Among these, the genome of D. vulgaris Hildenborough encodes a large number of two component systems consisting of 72 putative response regulators (RR) and 64 putative histidinekinases (HK), the majority of which are uncharacterized. We classified the D. vulgaris Hildenborough RRs based on their output domains and compared the distribution of RRs in other sequenced Desulfovibrio species. We have successfully purified most RRs and several HKs as His-tagged proteins. We performed phospho-transfer experiments to verify relationships between cognate pairs of HK and RR, and we have also mapped a few non-cognate HK-RR pairs. Presented here are our discoveries from the Desulfovibrio RR categorization and results from the in vitro studies using purified His tagged D. vulgaris HKs and RRs.