Analysis of Illumina Microbial Assemblies

Clum, Alicia ; Foster, Brian ; Froula, Jeff ; LaButti, Kurt ; Sczyrba, Alex ; Lapidus, Alla ; Woyke, Tanja

Lawrence Berkeley National Laboratory

关键词: Dna Sequencing;    Microorganisms;    Comparative Evaluations;    59;    Economics;   

DOI  :  10.2172/985368
RP-ID  :  LBNL-3671E-Poster
RP-ID  :  DE-AC02-05CH11231
RP-ID  :  985368

美国|英语

来源: UNT Digital Library

【 摘 要 】

Since the emerging of second generation sequencing technologies, the evaluation of different sequencing approaches and their assembly strategies for different types of genomes has become an important undertaken. Next generation sequencing technologies dramatically increase sequence throughput while decreasing cost, making them an attractive tool for whole genome shotgun sequencing. To compare different approaches for de-novo whole genome assembly, appropriate tools and a solid understanding of both quantity and quality of the underlying sequence data are crucial. Here, we performed an in-depth analysis of short-read Illumina sequence assembly strategies for bacterial and archaeal genomes. Different types of Illumina libraries as well as different trim parameters and assemblers were evaluated. Results of the comparative analysis and sequencing platforms will be presented. The goal of this analysis is to develop a cost-effective approach for the increased throughput of the generation of high quality microbial genomes.