Pseudo-stationary separation materials for highly parallel separations.

Singh, Anup K. ; Palmer, Christopher (University of Montana, Missoula, MT)

Sandia National Laboratories

关键词: Polymers.;    Polymers Amino Acids.;    Amino Acids;    Fluorescence;    Chromatography;   

DOI  :  10.2172/923083
RP-ID  :  SAND2005-2015
RP-ID  :  AC04-94AL85000
RP-ID  :  923083

美国|英语

来源: UNT Digital Library

【 摘 要 】

Goal of this study was to develop and characterize novel polymeric materials as pseudostationary phases in electrokinetic chromatography. Fundamental studies have characterized the chromatographic selectivity of the materials as a function of chemical structure and molecular conformation. The selectivities of the polymers has been studied extensively, resulting in a large body of fundamental knowledge regarding the performance and selectivity of polymeric pseudostationary phases. Two polymers have also been used for amino acid and peptide separations, and with laser induced fluorescence detection. The polymers performed well for the separation of derivatized amino acids, and provided some significant differences in selectivity relative to a commonly used micellar pseudostationary phase. The polymers did not perform well for peptide separations. The polymers were compatible with laser induced fluorescence detection, indicating that they should also be compatible with chip-based separations.