Assessment of disinfectants in explosive destruction system for biological agent destruction : LDRD final report FY04.

Simmons, Blake Alexander ; Didlake, John E. Jr. ; Bradshaw, Robert W. ; Crooker, Paul J. ; Buffleben, George M.

Sandia National Laboratories

关键词: Biological Weapons.;    Spores, Bacterial-Physiology;    Decontamination;    Bacillus;    59 Basic Biological Sciences;   

DOI  :  10.2172/920740
RP-ID  :  SAND2004-6303
RP-ID  :  AC04-94AL85000
RP-ID  :  920740

美国|英语

来源: UNT Digital Library

【 摘 要 】

Treatment systems that can neutralize biological agents are needed to mitigate risks from novel and legacy biohazards. Tests with Bacillus thuringiensis and Bacillus steurothemophilus spores were performed in a 190-liter, 1-112 lb TNT equivalent rated Explosive Destruction System (EDS) system to evaluate its capability to treat and destroy biological agents. Five tests were conducted using three different agents to kill the spores. The EDS was operated in steam autoclave, gas fumigation and liquid decontamination modes. The first three tests used EDS as an autoclave, which uses pressurized steam to kill the spores. Autoclaving was performed at 130-140 deg C for up to 2-hours. Tests with chlorine dioxide at 750 ppm concentration for 1 hour and 10% (vol) aqueous chlorine bleach solution for 1 hour were also performed. All tests resulted in complete neutralization of the bacterial spores based on no bacterial growth in post-treatment incubations. Explosively opening a glass container to expose the bacterial spores for treatment with steam was demonstrated and could easily be done for chlorine dioxide gas or liquid bleach.