Highly specific electronic signal transduction mediated by DNA/metal self-assembly.

Dentinger, Paul M. ; Pathak, Srikant

Sandia National Laboratories

关键词: Dna;    Self-Assembly.;    Oligonucleotides;    59 Basic Biological Sciences;    Dna-Chemistry.;   

DOI  :  10.2172/918237
RP-ID  :  SAND2003-8785
RP-ID  :  AC04-94AL85000
RP-ID  :  918237

美国|英语

来源: UNT Digital Library

【 摘 要 】

Highly specific interactions between DNA could potentially be amplified if the DNA interactions were utilized to assemble large scale parts. Fluidic assembly of microsystem parts has the potential for rapid and accurate placement of otherwise difficult to handle pieces. Ideally, each part would have a different chemical interaction that allowed it to interact with the substrate only in specific areas. One easy way to obtain a multiple chemical permutations is to use synthetic DNA oligomers. Si parts were prepared using silicon-on-insulator technology microfabrication techniques. Several surface chemistry protocols were developed to react commercial oligonucleotides to the parts. However, no obvious assembly was achieved. It was thought that small defects on the surface did not allow the microparts to be in close enough proximity for DNA hybridization, and this was. in part, confirmed by interferometry. To assist in the hybridization, plastic, pliable parts were manufactured and a new chemistry was developed. However, assembly was still absent even with the application of force. It is presently thought that one of three mechanisms is preventing the assembly. The surfaces of the two solid substrates can not get in close enough proximity, the surface chemistry lacks sufficient density to keep the parts from separating, or DNA interactions in close proximity on solid substrates are forbidden. These possibilities are discussed in detail.