Self-assembling holographic biosensors and biocomputers.

Light, Yooli Kim ; Bachand, George David (Sandia National Laboratories, Albuquerque, NM) ; Schoeniger, Joseph S. ; Trent, Amanda M. (Sandia National Laboratories, Albuquerque, NM)

Sandia National Laboratories

关键词: Polyethylene Glycols;    Self-Assembly.;    Diffraction;    Biosensors.;    59 Basic Biological Sciences;   

DOI  :  10.2172/884749
RP-ID  :  SAND2006-3244
RP-ID  :  AC04-94AL85000
RP-ID  :  884749

美国|英语

来源: UNT Digital Library

【 摘 要 】

We present concepts for self-assembly of diffractive optics with potential uses in biosensors and biocomputers. The simplest such optics, diffraction gratings, can potentially be made from chemically-stabilized microtubules migrating on nanopatterned tracks of the motor protein kinesin. We discuss the fabrication challenges involved in patterning sub-micron-scale structures with proteins that must be maintained in aqueous buffers to preserve their activity. A novel strategy is presented that employs dry contact printing onto glass-supported amino-silane monolayers of heterobifunctional crosslinkers, followed by solid-state reactions of these cross-linkers, to graft patterns of reactive groups onto the surface. Successive solution-phase addition of cysteine-mutant proteins and amine-reactive polyethylene glycol allows assembly of features onto the printed patterns. We present data from initial experiments showing successful micro- and nanopatterning of lines of single-cysteine mutants of kinesin interleaved with lines of polyethylene, indicating that this strategy can be employed to arrays of features with resolutions suitable for gratings.