Bioagent detection using miniaturized NMR and nanoparticle amplification : final LDRD report.

Clewett, C. F. M. ; Adams, David Price ; Fan, Hongyou ; Williams, John D. ; Sillerud, Laurel O. ; Alam, Todd Michael ; Aldophi, Natalie L. (New Mexico Resonance, Albuquerque, NM) ; McDowell, Andrew F. (New Mexico Resonance, Albuquerque, NM)

Sandia National Laboratories

关键词: Spectrometers;    Biological Warfare Agents;    98 Nuclear Disarmament, Safeguards, And Physical Protection;    Sensitivity;    Performance Testing;   

DOI  :  10.2172/896855
RP-ID  :  SAND2006-6931
RP-ID  :  AC04-94AL85000
RP-ID  :  896855

美国|英语

来源: UNT Digital Library

【 摘 要 】

This LDRD program was directed towards the development of a portable micro-nuclear magnetic resonance ({micro}-NMR) spectrometer for the detection of bioagents via induced amplification of solvent relaxation based on superparamagnetic nanoparticles. The first component of this research was the fabrication and testing of two different micro-coil ({micro}-coil) platforms: namely a planar spiral NMR {micro}-coil and a cylindrical solenoid NMR {micro}-coil. These fabrication techniques are described along with the testing of the NMR performance for the individual coils. The NMR relaxivity for a series of water soluble FeMn oxide nanoparticles was also determined to explore the influence of the nanoparticle size on the observed NMR relaxation properties. In addition, The use of commercially produced superparamagnetic iron oxide nanoparticles (SPIONs) for amplification via NMR based relaxation mechanisms was also demonstrated, with the lower detection limit in number of SPIONs per nanoliter (nL) being determined.