Identification of Metal Reductases using Proteomic Analysis

Lipton, Mary

Pacific Northwest National Laboratory (U.S.)

关键词: Microorganisms;    Oxidoreductases;    Enzymes;    Bioremediation;    59 Basic Biological Sciences;   

DOI  :  10.2172/896018
RP-ID  :  ERSD-1022403-2006
RP-ID  :  None
RP-ID  :  896018

美国|英语

来源: UNT Digital Library

【 摘 要 】

Central to the NABIR goal to develop the scientific basis for in situ remediation of radioactive contaminants is the fundamental understanding of microorganisms with dissimilatory metal reducing activity. In order to effectively exploit these bacteria, it is necessary to know which enzymes and pathways are involved. Additionally, it would be advantageous to understand the similarities and differences of these pathways across different bacteria for effective deployment in bioremediation, as well as to identify new microbes capable of such activities. Most approaches to identify these enzymes or enzyme complexes rely on biochemical purification to homogeneity with subsequent Nterminal sequencing of digested peptides. However, loss of activity before achieving purity often necessitates repetition of the entire process. Newly developed proteomics capabilities at PNNL allow for the identification of many proteins from a single sample through mass spectrometry analysis.