Anaerobic Biotransformation and Mobility of Pu and PuEDTA

Xun, Luying

Washington State University, Vancouver, WA

关键词: Enzymes;    Genes;    59 Basic Biological Sciences;    38 Radiation Chemistry, Radiochemistry, And Nuclear Chemistry;    Hypothesis;   

DOI  :  10.2172/893450
RP-ID  :  NABIR-1025342-2005
RP-ID  :  None
RP-ID  :  893450

美国|英语

来源: UNT Digital Library

【 摘 要 】

The objective of this report is to isolate anaerobic EDTA-degrading bacteria. Although our goal is to isolate anaerobic EDTA degraders, we initiated the experiments to include nitrilotriacetate (NTA), which is a structure homologue of EDTA. All the aerobic EDTA degraders can degrade NTA, but the isolated NTA degraders cannot degrade EDTA. Since NTA is a simpler structure homologue, it is likely that EDTA-degrading ability is evolved from NTA degradation. This hypothesis is further supported from our characterization of EDTA and NTA-degrading enzymes and genes (J. Bact. 179:1112-1116; and Appl. Environ. Microbiol. 67:688-695). The EDTA monooxygenase and NTA monooxygenase are highly homologous. EDTA monooxygenase can use both EDTA and NTA as substrates, but NTA monooxygenase can only use NTA as a substrate. Thus, we put our effort to isolate both NTA and EDTA degraders. In case, an anaerobic EDTA degrader is not immediately enriched, we will try to evolve the NTA degraders to use EDTA. Both aerobic and anaerobic enrichment cultures were set.