Examining microarray slide quality for the EPA using SNL's hyperspectral microarray scanner.

Rohde, Rachel M. ; Timlin, Jerilyn Ann

Sandia National Laboratories

关键词: Data Analysis;    Multivariate Analysis;    54 Environmental Sciences;    99 General And Miscellaneous//Mathematics, Computing, And Information Science;    Us Epa;   

DOI  :  10.2172/875988
RP-ID  :  SAND2005-7536
RP-ID  :  AC04-94AL85000
RP-ID  :  875988

美国|英语

来源: UNT Digital Library

【 摘 要 】

This report summarizes research performed at Sandia National Laboratories (SNL) in collaboration with the Environmental Protection Agency (EPA) to assess microarray quality on arrays from two platforms of interest to the EPA. Custom microarrays from two novel, commercially produced array platforms were imaged with SNL's unique hyperspectral imaging technology and multivariate data analysis was performed to investigate sources of emission on the arrays. No extraneous sources of emission were evident in any of the array areas scanned. This led to the conclusions that either of these array platforms could produce high quality, reliable microarray data for the EPA toxicology programs. Hyperspectral imaging results are presented and recommendations for microarray analyses using these platforms are detailed within the report.