Genomic and Genetic Approaches to Solvent Tolerance

Papoutsakis, Eleftherios T.

Northwestern University (Evanston, Ill.)

关键词: Genetics;    09 Biomass Fuels;    Solvents;    Hypothesis;    Engineers;   

DOI  :  10.2172/840821
RP-ID  :  NONE
RP-ID  :  FG36-03GO13160
RP-ID  :  840821

美国|英语

来源: UNT Digital Library

【 摘 要 】

The proposed research is to understand and exploit the molecular basis that determines tolerance of the industrially important anaerobic clostridia to solvents. Furthermore, we aim to develop general genomic and metabolic engineering strategies for understanding the molecular basis of tolerance to chemicals and for developing tolerant strains. Our hypothesis is that the molecular basis of what makes bacterial cells able to withstand high solvent concentrations can be used to metabolically engineer cells so that they can tolerate higher concentrations of solvents and related chemicals.