The spectrum of mutation produced by low dose radiation

Morley, Alexander A. ; Turner, David R.

Flinders University, Adelaide, SA (AU)

关键词: Dna;    Genetics;    Sensitivity Mutation;    Neoplasms;    59 Basic Biological Sciences;   

DOI  :  10.2172/834002
RP-ID  :  DOE/ER/62906- Final Report
RP-ID  :  FG02-00ER62906
RP-ID  :  834002

美国|英语

来源: UNT Digital Library

【 摘 要 】

Inherited mutations are the basis of evolution and acquired mutations in humans are important in ageing, cancer and possibly various forms of tissue degeneration. Mutations are responsible for many of the long-term effects of radiation. However, sensitive direct detection of mutations in humans has been difficult. The aims of the project were to develop methods for the sensitive enumeration of mutations in DNA, to measure mutation frequencies in a wide variety of tissue types and to quantify the mutational effect of direct oxidative damage produced by radiation, at both high and low doses. The project was successful in developing a sensitive method which could detect mutations directly in the genetic material, DNA at a sensitivity of 1 mutated molecule in 1000000000 unmutated molecules. However a number of methodological problems had to be overcome and lack of ongoing funding made it impossible to fulfill all of the aims of the project