A hereditary disposition for bovine peripheral nerve sheath tumors in Danish Holstein cattle

Acta Veterinaria Scandinavica

Anette B Grossi⁴  Jørgen S Agerholm²  Knud Christensen⁵  Henrik E Jensen¹  Páll S Leifsson¹  Christian Bendixen³  Peter Karlskov-Mortensen⁵  Merete Fredholm⁵ 

[1] Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Ridebanevej 3, Frederiksberg C, 1870, Denmark

[2] Department of Large Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 68, Frederiksberg C, 1870, Denmark

[3] Department of Molecular Biology and Genetics, Faculty of Science and Technology, University of Aarhus, Blichers Allé 20, Tjele, 8830, Denmark

[4] Present address: Ellegaard Göttingen Minipigs A/S, Sorø Landevej 302, Dalmose, 4261, Denmark

[5] Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 3, Frederiksberg C, 1870, Denmark

关键词: Schwannoma;    Neurofibromatosis;    Neurofibroma;    Neoplasms;    Genome wide association study;    Genetics;    Cattle;   

Others  :  1082517
DOI  :  10.1186/s13028-014-0085-8

 received in 2013-11-25, accepted in 2014-12-02,  发布年份 2014

【 摘 要 】

Background

Peripheral nerve sheath tumors (PNSTs) are frequently found in Danish cattle at slaughter. Bovine PNSTs share several gross and histopathological characteristics with the PNSTs in humans with heritable neurofibromatosis syndromes. The aim of the present study was to investigate a possible hereditary disposition to PNSTs in dairy cattle by statistical analysis performed on data from 567 cattle with PNSTs. Furthermore, a preliminary genome-wide association study (GWAS) was performed on DNA isolated from 28 affected and 28 non-affected Holstein cows to identify loci in the bovine genome involved in the development of PNSTs.

Results

PNSTs were significantly more common in the Danish Holstein breed than in other breeds with 0.49% of Danish Holsteins slaughtered during an eight-year-period having PNSTs. PNSTs also occurred significantly more frequently in the offspring of some specific Holstein sires. Examination of three generation pedigrees showed that these sires were genetically related through a widely used US Holstein sire. The PNSTs included in GWAS were histologically classified as neurofibroma-schwannoma (43%), schwannoma (36%) and neurofibroma (21%) and derived from Holstein cows with multiple PNSTs. A single SNP on chromosome 27 reached genome-wide significance.

Conclusions

Gross and histological characteristics of bovine PNSTs are comparable to PNSTs in humans (schwannomatosis). Danish Holsteins are genetically disposed to develop PNSTs but the examined materials are insufficient to allow determination of the mode of inheritance.

【 授权许可】

   
2014 Grossi et al.; licensee BioMed Central Ltd.

【 图 表 】

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