Monitoring Genetic and Metabolic Potential for In-Situ Bioremediation: Mass Spectrometry

Buchanan, Michelle V. ; Britt, Phillip F. ; Doktycz, Mitchel J. ; Hurst, Gregory B. ; Lidstrom, Mary E.

Oak Ridge National Laboratory

关键词: 63 Radiation, Thermal, And Other Environmental Pollutant Effects On Living Organisms And Biological Materials;    Dna;    54 Environmental Sciences;    Genes;    Bioremediation;   

DOI  :  10.2172/827403
RP-ID  :  EMSP-55108--2000
RP-ID  :  827403

美国|英语

来源: UNT Digital Library

【 摘 要 】

A number of DOE sites are contaminated with dense non-aqueous phase liquids (DNAPLs) such as carbon tetrachloride and trichloroethylene. At many of these sites, microbial bioremediation is an attractive strategy for cleanup, since it has the potential to degrade DNAPLs in situ. A rapid screening method to determine the broad range potential of a site's microbial population for contaminant degradation would greatly facilitate assessment for in situ bioremediation, as well as for monitoring ongoing bioremediation treatment. Current laboratory-based treatability methods are cumbersome and expensive. In this project, we are developing methods based on matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for rapid and accurate detection of polymerase chain reaction (PCR) products from microbial genes involved in biodegradation of pollutants. PCR primers are being developed to amplify DNA sequences that are amenable to MALDI-MS detection. This work will lay the foundation for development of a field-portable MS-based technique for rapid on site assessment and monitoring of bioremediation processes.