Chlorinated Hydrocarbon Degradation in Plants: Mechanisms and Enhancement of Phytoremediation of Groundwater Contamination

Strand, Stuart E.

University of Washington

关键词: 63 Radiation, Thermal, And Other Environmental Pollutant Effects On Living Organisms And Biological Materials;    Cytochromes;    54 Environmental Sciences;    Enzymes;    Genes;   

DOI  :  10.2172/834670
RP-ID  :  EMSP-73858-2002
RP-ID  :  FG07-96ER20256
RP-ID  :  834670

美国|英语

来源: UNT Digital Library

【 摘 要 】

Several varieties of transgenic poplar containing cytochrome P-450 2E1 have been constructed and are undergoing tests. Strategies for improving public acceptance and safety of transgenic poplar for chlorinated hydrocarbon phytoremediation are being developed. We have discovered a unique rhizobium species that lives within the stems of poplar and we are investigating whether this bacterium contributes nitrogen fixed from the air to the plant and whether this endophyte could be used to introduce genes into poplar. Studies of the production of chloride ion from TCE have shown that our present P-450 constructs did not produce chloride more rapidly than wild type plants. Follow-up studies will determine if there are other rate limiting downstream steps in TCE metabolism in plants. Studies of the metabolism of carbon tetrachloride in poplar cells have provided evidence that the native plant metabolism is due to the activity of oxidative enzymes similar to the mammalian cytochrome P-450 2E1.