Automated cloning methods.

Collart, F.

Argonne National Laboratory

关键词: Storage;    Molecular Biology;    59 Basic Biological Sciences;    Robots;    Automation;   

DOI  :  10.2172/786915
RP-ID  :  ANL/BIO/01-1
RP-ID  :  W-31-109-ENG-38
RP-ID  :  786915

美国|英语

来源: UNT Digital Library

【 摘 要 】

Argonne has developed a series of automated protocols to generate bacterial expression clones by using a robotic system designed to be used in procedures associated with molecular biology. The system provides plate storage, temperature control from 4 to 37 C at various locations, and Biomek and Multimek pipetting stations. The automated system consists of a robot that transports sources from the active station on the automation system. Protocols for the automated generation of bacterial expression clones can be grouped into three categories (Figure 1). Fragment generation protocols are initiated on day one of the expression cloning procedure and encompass those protocols involved in generating purified coding region (PCR).